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Santa Cruz Biotechnology rank
Rank, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 275 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+rank/RANK+Antibody/pm40783844-91-17-24
Average 94 stars, based on 275 article reviews
rank - by Bioz Stars, 2026-10
94/100 stars

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Related Articles

Saline:

Article Title: B-cell intrinsic RANK signaling cooperates with TCL1 to induce lineage-dependent B-cell transformation
Article Snippet: .. Membranes were blocked for 1 h in 5 % BSA in 58 tris-buffered saline with Tween 20 (TBST, 0.1 % Tween 20) and probed with the following 59 primary and secondary antibodies: anti- actin (#60008-I-Ig, Proteintech), anti-AKT (#9272, 60 Cell Signaling), anti-ERK (#9102, Cell Signaling), anti-pAKT (#4056, Cell Signaling ), anti-61 pERK (#4370, Cell Signaling), anti-pJNK (#9251, Cell Signaling), anti-JNK (#sc-7345, Santa 62 Cruz), anti-RANK (#sc-374360, Santa Cruz), anti-pSRC family (#2101, Cell Signaling), anti-63 SRC (2110, Cell Signaling), anti-pPLC2 (#3871, Cell Signaling), anti-PLC2 (#3872, Cell 64 Signaling), anti-pIkk/ (#2078, Cell Signaling), anti-Ikk (#2682, Cell Signaling) and anti- 65 tubulin (#12351, Cell signaling), anti-rabbit IgG-HRP (#7074 Cell Signaling) and anti-mouse 66 IgG (#7076, Cell Signaling). ..

Polyacrylamide Gel Electrophoresis:

Article Title: A20 controls RANK-dependent osteoclast formation and bone physiology.
Article Snippet: The anti-inflammatory protein A20 serves as a critical brake on NF–jB signaling and NF–jB-dependent inflammation.. In humans, polymorphisms in or near the TNFAIP3/A20 gene have been associated with several inflammatory disorders, including rheumatoid arthritis (RA), and experimental studies in mice have demonstrated that myeloid-specific A20 deficiency causes the development of a severe polyarthritis resembling human RA.. Myeloid A20 deficiency also promotes osteoclastogenesis in mice, suggesting a role for A20 in the regulation of osteoclast differentiation and bone formation.

Semi Dry Blot:

Article Title: A20 controls RANK-dependent osteoclast formation and bone physiology.
Article Snippet: The anti-inflammatory protein A20 serves as a critical brake on NF–jB signaling and NF–jB-dependent inflammation.. In humans, polymorphisms in or near the TNFAIP3/A20 gene have been associated with several inflammatory disorders, including rheumatoid arthritis (RA), and experimental studies in mice have demonstrated that myeloid-specific A20 deficiency causes the development of a severe polyarthritis resembling human RA.. Myeloid A20 deficiency also promotes osteoclastogenesis in mice, suggesting a role for A20 in the regulation of osteoclast differentiation and bone formation.

Formalin-fixed Paraffin-Embedded:

Article Title: Downregulation of osteoprotegerin in colorectal cancer cells promotes liver metastasis via activating tumor-associated macrophage
Article Snippet: .. For IF, FFPE human sections or 4% paraformaldehyde-fixed mouse cryosections were incubated with anti-CD68, anti-CD206, anti-RANK (Santa Cruz, H-7, [1:50]), anti-mouse F4/80 (eBioscience, BM8, [1:200]) or anti-mouse CD206 (Bio-Rad, MR5D3, [1:100]) followed by incubation with a secondary antibody. .. Migration of dTHP-1 cells was assessed using 24-well Transwell cell culture chambers (5 μm-pore membrane) (CORNING, Corning, NY, USA).

Article Title: Downregulation of osteoprotegerin in colorectal cancer cells promotes liver metastasis via activating tumor-associated macrophage.
Article Snippet: .. For IF, FFPE human sections or 4% paraformaldehydefixed mouse cryosections were incubated with anti-CD68, anti-CD206, anti-RANK (Santa Cruz, H-7, [1:50]), anti-mouse F4/80 (eBioscience, BM8, [1:200]) or anti-mouse CD206 (Bio-Rad, MR5D3, [1:100]) followed by incubation with a secondary antibody. .. Migration of dTHP-1 cells was assessed using 24-well Transwell cell culture chambers (5 μm-pore membrane) (CORNING, Corning, NY, USA).

Incubation:

Article Title: Downregulation of osteoprotegerin in colorectal cancer cells promotes liver metastasis via activating tumor-associated macrophage
Article Snippet: .. For IF, FFPE human sections or 4% paraformaldehyde-fixed mouse cryosections were incubated with anti-CD68, anti-CD206, anti-RANK (Santa Cruz, H-7, [1:50]), anti-mouse F4/80 (eBioscience, BM8, [1:200]) or anti-mouse CD206 (Bio-Rad, MR5D3, [1:100]) followed by incubation with a secondary antibody. .. Migration of dTHP-1 cells was assessed using 24-well Transwell cell culture chambers (5 μm-pore membrane) (CORNING, Corning, NY, USA).

Article Title: Downregulation of osteoprotegerin in colorectal cancer cells promotes liver metastasis via activating tumor-associated macrophage.
Article Snippet: .. For IF, FFPE human sections or 4% paraformaldehydefixed mouse cryosections were incubated with anti-CD68, anti-CD206, anti-RANK (Santa Cruz, H-7, [1:50]), anti-mouse F4/80 (eBioscience, BM8, [1:200]) or anti-mouse CD206 (Bio-Rad, MR5D3, [1:100]) followed by incubation with a secondary antibody. .. Migration of dTHP-1 cells was assessed using 24-well Transwell cell culture chambers (5 μm-pore membrane) (CORNING, Corning, NY, USA).

Article Title: Microbial chaperonin 60 inhibits osteoclast differentiation by interfering with RANK/RANKL binding and overexpression of lipocalin2.
Article Snippet: .. Subsequently, the cells were blocked with 5 % bovine serum albumin (BSA) in PBS containing 0.5 % Tween 20 (PBS-T) at room temperature for 30 min and incubated with the following primary antibodies: anti-RANK (1:100 dilution, sc-374,360; Santa Cruz Biotechnology), anti-RANKL (1:100 dilution, AF462; R&D Systems), and anti-GroEL (1:100 dilution, G6532; Sigma) at room temperature for 1 h; all antibodies were diluted in 1 % BSA in PBS-T. .. The cells were washed thrice with PBS-T for 5 min and then incubated with secondary antibodies [donkey anti-mouse IgG Alexa 546 (A10036; yellow), donkey anti-goat IgG Alexa 488 (A11055; green), and donkey anti-rabbit Alexa 647 (A31573; red); 1:500 diluted in 1 % BSA in PBS-T and all acquired from Thermo Fisher Scientific] at room temperature for 1 h. Nuclei were counterstained with DAPI (1 μg/ml, D9542; Sigma) for 10 min, and the coverslips were mounted with ProLong gold antifade mountant (Invitrogen, Carlsbad, California).

Article Title: Effect of systemic administration of Bifidobacterium animalis subsp. lactis HN019 on apical periodontitis.
Article Snippet: .. The slides were then incubated overnight under refrigeration with the primary antibodies diluted in 1% BSA: anti-RANK (polyclonal rabbit antibody, Santa Cruz Biotechnology Inc, Santa Cruz, USA; 1:700 dilution) and antiRANKL (polyclonal goat antibody, Santa Cruz Biotechnology Inc, 1:400 dilution). ..

other:

Article Title: Upregulated bone morphogenetic protein 8A (BMP8A) in triple negative breast cancer (TNBC) and its involvement in the bone metastasis
Article Snippet: Anti-RANK , Rabbit , 66, 56.4, 36.3 , Santa Cruz , sc9072 , 1:1,000.



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EA can inhibit the activation of microglia in the pPD stage mediated by TLR2. ( A ) Western blot detection of TLR2 protein expression in the substantia nigra. ( B ) Western blot detection of MyD88 protein expression in the substantia nigra. ( C ) Western blot detection of p-NF-κB-p65 expression in the substantia nigra. ( D ) Western blot detection of NLRP3 protein expression in the substantia nigra. ( E ) Western blot detection of Caspase-1 protein expression in the substantia nigra. ( F ) Western blot detection of GSDMD protein expression in the substantia nigra. ( G ) Western blot detection of IL-1β protein expression in the substantia nigra. (n=6; compared with the control group, *** P <0.001; compared with the model group, ### P <0.001, ## P <0.01, # P <0.05).

Journal: Journal of Inflammation Research

Article Title: Electroacupuncture Inhibits the Early Neuroinflammatory Cascade Triggered by TLR2 in the Prodromal Period of PD

doi: 10.2147/JIR.S585729

Figure Lengend Snippet: EA can inhibit the activation of microglia in the pPD stage mediated by TLR2. ( A ) Western blot detection of TLR2 protein expression in the substantia nigra. ( B ) Western blot detection of MyD88 protein expression in the substantia nigra. ( C ) Western blot detection of p-NF-κB-p65 expression in the substantia nigra. ( D ) Western blot detection of NLRP3 protein expression in the substantia nigra. ( E ) Western blot detection of Caspase-1 protein expression in the substantia nigra. ( F ) Western blot detection of GSDMD protein expression in the substantia nigra. ( G ) Western blot detection of IL-1β protein expression in the substantia nigra. (n=6; compared with the control group, *** P <0.001; compared with the model group, ### P <0.001, ## P <0.01, # P <0.05).

Article Snippet: The membrane was incubated overnight at 4°C with primary antibodies specific to TLR2 (#bs-1019R, Bioss, 1:1000), MyD88 (#bs-1047R, Bioss, 1:1000), p-NF-κB-p65 (#bs-7343R, Bioss, 1:1000), NLRP3 (#bs-41293R, Bioss, 1:1000), caspase-1 (#bsm-52441R, Bioss, 1:1000), IL-1β (#bs-0812R, Bioss, 1:1000), and GSDMD (#bs-14287R, Bioss, 1:1000), followed by incubation with an IgG secondary antibody (#bs-10900R, Bioss, 1:5000) for 1 hour in the dark.

Techniques: Activation Assay, Western Blot, Expressing, Control

EA intervention affects the expression of TLR2 in different regions of pPD, inhibits microglial activation, regulates the TLR2/NF-κB/NLRP3 pathway, and suppresses pyroptosis of microglia.

Journal: Journal of Inflammation Research

Article Title: Electroacupuncture Inhibits the Early Neuroinflammatory Cascade Triggered by TLR2 in the Prodromal Period of PD

doi: 10.2147/JIR.S585729

Figure Lengend Snippet: EA intervention affects the expression of TLR2 in different regions of pPD, inhibits microglial activation, regulates the TLR2/NF-κB/NLRP3 pathway, and suppresses pyroptosis of microglia.

Article Snippet: The membrane was incubated overnight at 4°C with primary antibodies specific to TLR2 (#bs-1019R, Bioss, 1:1000), MyD88 (#bs-1047R, Bioss, 1:1000), p-NF-κB-p65 (#bs-7343R, Bioss, 1:1000), NLRP3 (#bs-41293R, Bioss, 1:1000), caspase-1 (#bsm-52441R, Bioss, 1:1000), IL-1β (#bs-0812R, Bioss, 1:1000), and GSDMD (#bs-14287R, Bioss, 1:1000), followed by incubation with an IgG secondary antibody (#bs-10900R, Bioss, 1:5000) for 1 hour in the dark.

Techniques: Expressing, Activation Assay